Pulmonary CD4+ cytokine responses in mice reveal differences in the relative immunogenicity of cold-adapted influenza A vaccine donor strains
Identifieur interne : 001647 ( Main/Exploration ); précédent : 001646; suivant : 001648Pulmonary CD4+ cytokine responses in mice reveal differences in the relative immunogenicity of cold-adapted influenza A vaccine donor strains
Auteurs : Mark D. Wareing [Australie] ; Leonard C. Harrison [Australie] ; Gregory A. Tannock [Australie]Source :
- Vaccine [ 0264-410X ] ; 2005.
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- Pascal (Inist)
- Wicri :
- topic : Vaccin.
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Abstract
We previously described differences in the 50% protective dose and isotype-specific antibody secreting cell (ASC) responses to US and Russian influenza A cold-adapted (ca) donor strains in the lungs of BALB/c mice [Wareing MD, Watson JM, Brooks MJ, Tannock GA. Immunogenic and isotype-specific responses to Russian and US cold-adapted influenza A vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57, and A/Ann Arbor/6/60 (H2N2) in mice. J Med Virol 2001;65(1):171-7]. A/Leningrad/134/17/57 (Len/17-ca) was shown to be a superior immunogen to A/Leningrad/I 34/47/57-ca (Len/47-ca), which, in turn, was superior to A/Ann Arbor/6/60-ca (AA-ca) but no other comparative data exist. In order to extend our findings and determine a means for selecting the most immunogenic ca influenza A vaccine, the intracellular cytokine responses by CD4+ and CD8+ T cells to AA-ca, Len/47-ca and Len/17-ca and their respective wild-type parental viruses were compared in mice. Day 5 after infection with Len/17-ca, when levels of IL-2, -4 and -10 were highest in the mediastinal lymph nodes (MLN) and lungs, was chosen as the optimum time to harvest lymphocytes and 72 h was determined to be the optimum restimulation period for lymphocytes by APCs. Under these conditions, the frequency of CD4+ and CD8+ cells expressing cytokines was highest in the lungs compared with the MLN. A dominant IL-6 response was induced, although all virus strains induced a Thl/Th2 cytokine profile. While the CD8+ cytokine response appeared non-specific, the cytokine response elicited in the lungs by CD4+ cells to Len/17-ca-inoculation was greater than that induced by Len/47-ca, or AA/ca. The CD4+ cytokine response in the lungs may be a useful measure of immunogenicity to determine the most effective influenza reassortant for inclusion in vaccines.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Pulmonary CD4<sup>+</sup> cytokine responses in mice reveal differences in the relative immunogenicity of cold-adapted influenza A vaccine donor strains</title><author><name sortKey="Wareing, Mark D" sort="Wareing, Mark D" uniqKey="Wareing M" first="Mark D." last="Wareing">Mark D. Wareing</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71</s1><s2>Bundoora, Vic. 3083</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Bundoora, Vic. 3083</wicri:noRegion></affiliation></author><author><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C." last="Harrison">Leonard C. Harrison</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Walter and Elisa Hall Institute of Medical Research</s1><s2>Melbourne, Vic</s2><s3>AUS</s3><sZ>2 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Walter and Elisa Hall Institute of Medical Research</wicri:noRegion></affiliation></author><author><name sortKey="Tannock, Gregory A" sort="Tannock, Gregory A" uniqKey="Tannock G" first="Gregory A." last="Tannock">Gregory A. Tannock</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71</s1><s2>Bundoora, Vic. 3083</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Bundoora, Vic. 3083</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0296341</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0296341 INIST</idno><idno type="RBID">Pascal:05-0296341</idno><idno type="wicri:Area/PascalFrancis/Corpus">000052</idno><idno type="wicri:Area/PascalFrancis/Curation">000022</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000051</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000051</idno><idno type="wicri:Area/Main/Merge">001675</idno><idno type="wicri:Area/Main/Curation">001647</idno><idno type="wicri:Area/Main/Exploration">001647</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Pulmonary CD4<sup>+</sup> cytokine responses in mice reveal differences in the relative immunogenicity of cold-adapted influenza A vaccine donor strains</title><author><name sortKey="Wareing, Mark D" sort="Wareing, Mark D" uniqKey="Wareing M" first="Mark D." last="Wareing">Mark D. Wareing</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71</s1><s2>Bundoora, Vic. 3083</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Bundoora, Vic. 3083</wicri:noRegion></affiliation></author><author><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C." last="Harrison">Leonard C. Harrison</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Walter and Elisa Hall Institute of Medical Research</s1><s2>Melbourne, Vic</s2><s3>AUS</s3><sZ>2 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Walter and Elisa Hall Institute of Medical Research</wicri:noRegion></affiliation></author><author><name sortKey="Tannock, Gregory A" sort="Tannock, Gregory A" uniqKey="Tannock G" first="Gregory A." last="Tannock">Gregory A. Tannock</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Biotechnology and Environmental Biology, RMIT University, P.O. Box 71</s1><s2>Bundoora, Vic. 3083</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Bundoora, Vic. 3083</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytokine</term><term>Immunogenicity</term><term>Influenza A</term><term>Mouse</term><term>Vaccine</term><term>Vaccine strain</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Souris</term><term>Cytokine</term><term>Immunogénicité</term><term>Souche vaccinale</term><term>Vaccin</term><term>Grippe A</term><term>Antigène CD4</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We previously described differences in the 50% protective dose and isotype-specific antibody secreting cell (ASC) responses to US and Russian influenza A cold-adapted (ca) donor strains in the lungs of BALB/c mice [Wareing MD, Watson JM, Brooks MJ, Tannock GA. Immunogenic and isotype-specific responses to Russian and US cold-adapted influenza A vaccine donor strains A/Leningrad/134/17/57, A/Leningrad/134/47/57, and A/Ann Arbor/6/60 (H2N2) in mice. J Med Virol 2001;65(1):171-7]. A/Leningrad/134/17/57 (Len/17-ca) was shown to be a superior immunogen to A/Leningrad/I 34/47/57-ca (Len/47-ca), which, in turn, was superior to A/Ann Arbor/6/60-ca (AA-ca) but no other comparative data exist. In order to extend our findings and determine a means for selecting the most immunogenic ca influenza A vaccine, the intracellular cytokine responses by CD4<sup>+</sup> and CD8<sup>+</sup> T cells to AA-ca, Len/47-ca and Len/17-ca and their respective wild-type parental viruses were compared in mice. Day 5 after infection with Len/17-ca, when levels of IL-2, -4 and -10 were highest in the mediastinal lymph nodes (MLN) and lungs, was chosen as the optimum time to harvest lymphocytes and 72 h was determined to be the optimum restimulation period for lymphocytes by APCs. Under these conditions, the frequency of CD4<sup>+</sup> and CD8<sup>+</sup> cells expressing cytokines was highest in the lungs compared with the MLN. A dominant IL-6 response was induced, although all virus strains induced a Thl/Th2 cytokine profile. While the CD8<sup>+</sup> cytokine response appeared non-specific, the cytokine response elicited in the lungs by CD4<sup>+</sup> cells to Len/17-ca-inoculation was greater than that induced by Len/47-ca, or AA/ca. The CD4<sup>+</sup> cytokine response in the lungs may be a useful measure of immunogenicity to determine the most effective influenza reassortant for inclusion in vaccines.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Wareing, Mark D" sort="Wareing, Mark D" uniqKey="Wareing M" first="Mark D." last="Wareing">Mark D. Wareing</name></noRegion><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C." last="Harrison">Leonard C. Harrison</name><name sortKey="Tannock, Gregory A" sort="Tannock, Gregory A" uniqKey="Tannock G" first="Gregory A." last="Tannock">Gregory A. Tannock</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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